5 Simple Techniques For hplc column selection
5 Simple Techniques For hplc column selection
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Huge variety of processes and flow path configurations accessible to structure your individual multi-stage process.
Liquid chromatography is one of the 3 major branches of chromatography. It involves a small volume of liquid sample placement into a tube packed with porous particles.
Be part of our webinar to know what can make monoliths perfect for the problems of purifying substantial biomolecules.
Sartorius chromatography consumables protect the total number of separation technologies and methodologies out there to accommodate any approach and any mo...
Manual columns together using a packing station offer an economic toolbox for column packing along with the capability to create and optimize column packing as essential for personal procedures and the flexibility.
Permits greater productivity than traditional chromatography, minimized buffer and resin volumes as well as reduce resin expenses
Agilent-certified provider gurus are located across the globe and manage an inventory of legitimate Agilent pieces. They’ll instantly diagnose your method onsite and carry out any vital repairs—ordinarily in just one take a look at.
The subsequent online video focusing solely regarding how to clean up your HPLC column by way of reverse flushing. It'd be time to flush your HPLC column after you’re looking at superior back again force, hplc column chemistry minimal performance, break up peaks, or the most common situation, particulate Construct-up at The pinnacle of the column.
Slender-bore columns (one–two mm) are utilized for programs when much more sensitivity is desired both with Unique UV-vis detectors, fluorescence detection or with other detection techniques like liquid chromatography-mass spectrometry
Even the most skilled researchers make the mistake of injecting the wrong samples onto columns, even though.
Related or semi-related downstream processing delivers considerable enhancements to productivity, Price, timelines, and facility footprint. Our flexible portfolio allows you decide on an intensification tactic that aligns more info with the exceptional approach aims.
Sartorius gurus signify considerable knowledge in chromatography and purification of biomolecules along with analytical methods improvement.
Trifluoroacetic acid (TFA) as additive to your cell section is greatly used for complicated mixtures of biomedical samples, mainly peptides and proteins, making use of primarily UV dependent detectors. They're hardly ever Utilized in mass spectrometry strategies, on account of residues it could possibly depart from the detector and solvent shipping process, which interfere Along with the Investigation and detection.
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